I want to request testing by your laboratory, what do I have to do for this?

You will need to contact your doctor. Please tell your doctor to visit our website and to download our test kit order form (USA or outside USA), fill and sign it and submit it to our office together with a copy of his/her medical license. After that we will send a test kit to your doctor’s office.

I am a doctor and it is the first time I am going to send you samples. Should I send you my medical license?

Yes, please submit a copy of your medical license to our office by fax or e-mail.

The instruction sheet for Nagalase says to stop immune stimulants prior to testing. However, these are part of the treatment for the patient. Will these immune stimulants affect the result if they are not stopped for testing?

The immune stimulants decrease the Nagalase activity. We advise to draw blood for the Nagalase test just before the immune stimulant medication is given. If this medication is given once a week, as is mostly the case, then this will pose no problem. However, if a one week interval is not possible: at least try to use the same time period each time to have the best follow up.

The instruction sheet for the Methylation Pathway Panel says to stop medication (if possible) prior to testing. What kind of medication should be discontinued and for what time?

It would be better to stop intake of vitamin B6 and B12 for 48 hours before the blood draw since the high intake of these vitamins may affect the results.

Methylation Pathway Panel – Health Diagnostics and Research Institute

A blood test for methylation needs to be done for any patient’s complete methylation testing.

Methylation is the process of controlled transfer of a methyl group (one carbon atom and three hydrogen atoms) onto amino acids, proteins, enzymes, and DNA in every cell and tissue of the body to regulate healing, cell energy, genetic expression of DNA, neurological function, liver detoxification, immunity, etc. This process is one of the essential metabolic functions of the body and is catalyzed by a variety of enzymes. Many case histories and several scientific articles have demonstrated that this methylation process is responsive to environmental conditions (toxic metals, organophosphates, infectious agents, etc.) and degrades with age, a process associated with a large variety of age-related disorders. Thus, with respect to the effect of methylation, it is a continuous struggle in life to adapt to the ever-changing environment. In fact, health and quality of life are highly dependent on the methylation process.

Methylation processes are central to the biochemical basis of the neuropsychiatry of folate and B12 metabolism.

The biochemical basis of the interrelationship between folate and cobalamine is the maintenance of two functions, nucleic acid synthesis and the methylation reactions. The latter is particularly important in the brain and relies especially on maintaining the concentration of S-adenosyl methionine (SAM), which, in turn, maintains the methylation reactions whose inhibition is considered to cause cobalamine deficiency-associated neuropathy. Defective methylation processes can lead to a number of serious health conditions. A simple abnormality in the methylation pathway, compounded with further assaults from environmental and infectious agents (including Borrelia), can lead to a wide range of conditions including cardiovascular disease, neurotransmitter imbalances, cancer, diabetes, abnormal immune function, chronic fatigue syndrome (CFS), multiple sclerosis (MS), cognitive dysfunction in patients with dementia, neurological and psychiatric disorders, Alzheimers disease (AD), Down syndrome, autism, neural tube defects, and chronic inflammation.

Methylation Pathway Parameters Tested:

Adenosine (plasma), Glutathione (oxidized and reduced) (plasma), Tetrahydrofolate (THF), 5-methyl-THF, 10-forrnyl-THF, 5-formyl-THF, Folic Acid (plasma), Folinic Acid(Whole Blood), Total Active Folate (RBC), S-adenosyl-methionine (RBC), S-adenosyl-homocysteine (RBC).

Extended Methylation Pathway Parameters Tested:

Adenosine (plasma), Glutathione (oxidized and reduced) (plasma), Tetrahydrofolate (THF), 5-methyl-THF, 10-formyl-THF, 5-formyl-THF, Folic Acid (plasma), Folinic Acid (Whole Blood), Total Active Folate (RBC), S-adenosyl-methionine (RBC), S-adenosyl-homocysteine (RBC), Methionine, Cysteine, Cystathionine, Taurine, and Homocysteine (plasma).

Ultimate Methylation Pathway Panel :